Proteomics

High resolution mass spectrometry (HR-MS) is a key technology that enables proteome research in the biomedical and clinical field. In-depth analyses of protein expression profiles, posttranslational protein modifications (PTMs) or protein/protein interaction networks provide deep insights into the molecular basis of health and disease. In addition, mass spectrometry offers promising new approaches to the development of molecular biomarkers for the diagnosis and treatment of severe diseases,

At the UMG’s Core Facility Proteomics (CF Pro), we offer a comprehensive portfolio of HR-MS-based workflows for the analysis of complex samples to both internal and external research groups. In addition to cell-based experimental approaches, we have developed and implemented robust label-free workflows for proteome analysis in a range of tissues and body fluids. These allow for the analysis of medium-sized sample cohorts as a prerequisite for clinical proteomics. In addition to our existing service portfolio for routine analysis, we are constantly developing novel, customized analytical workflows on a collaborative basis. Please approach us to find out more!

Organizationally, the Core Facility is associated with the Bioanalytics Research Group led by Prof. Dr. Henning Urlaub at the UMG’s Department of Clinical Chemistry

Equipment

Hardware

  • Hybrid quadrupole/orbitrap mass spectrometer Q Exactive (Thermo Fisher Scientific)
  • Hybrid ion mobility/quadrupole/time-of-flight mass spectrometer timsTOF Pro 2 (Bruker)
  • Pressure Cycler Barocycler 2320 EXT (Pressure Biosciences) for tissue lysis
  • Chromatography system Äkta Pure 25 (Cytiva) for peptide and protein separations
  • Mini-gel system Xcell SureLock (Invitrogen) for SDS-PAGE and BN-PAGE


Software

  • Spectronaut 15.1 (Biognosys)
  • MaxQuant 2.0.3, Perseus 1.6.15.0 (MPI für Biochemie)
  • MASCOT 2.7 (Matrixscience)
  • Peaks X Studio (Bioinformatics Solutions)
  • ProteinPilot 5.0, PeakView 2.2 (SCIEX)
  • Scaffold 5 (Proteome Software)
  • Skyline 22.0 (University of Washington)

Our services

The Core Facility Proteomics offers a variety of techniques for proteomics sample preparation, including e.g. protein precipitation, SDS-PAGE, enzymatic digestion, and chromatographic peptide and protein separations. We have established capabilities for the routine, parallelized lysis and cleanup of tissue samples.

For HR-MS analysis we operate two high resolution hybrid mass spectrometers with quadrupole/orbitrap and quadrupole/time-of-flight mass analyzers. These enable a range of workflows including data-dependent acquisition (DDA), data-independent acquisition (DDA) and parallel reaction monitoring (PRM) mass spectrometry. Customized normalization strategies allow for both relative and absolute protein quantitation.

Our services include in-depth consultancy and project qualification; data processing and first-level statistical analysis; and finally a detailed, personalized communication of the obtained results. We additionally support the UMG’s research groups in funding applications via detailed cost estimates and letters of support.

Contact

Our service portfolio as well as our user documentation are frequently extended and updated. Please find the current documents at the bottom of this page. Do not hesitate to contact us for inquiries and project discussions!

Facility Head

Dr. Christof Lenz

Dr. Christof Lenz

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Scientific Supervision

Prof. Dr. Henning Urlaub

Prof. Dr. Henning Urlaub

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Technical Assistance

Lisa Neuenroth

 Lisa Neuenroth

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