Clinical Optical Microscopy
3D imaging of tissue blocks using mesoscopic light sheet microscopy
Using mesoscopic light sheet microscopy, we can tomographically image intact tissue blocks of approx. 1 x 1 cm. In our BMBF-funded project “3Dpatho”, a microscope system is being established for use in everyday pathology. Combined with customized clearing and fluorescence staining protocols, it is now possible to perform tomographic imaging with the largest possible field size and penetration depth at cellular resolution.
Deep-tissue near-infrared imaging in tissue slices
Imaging in the near-infrared spectral window offers the advantage of deeper tissue penetration. We apply new near-infrared sample preparation and staining protocols in combination with confocal microscopy in the near-infrared spectrum (800-1000 nm).
Molecular imaging by Fluorescence Lifetime Imaging Microscopy (FLIM)
We use Fluorescence Lifetime Imaging Microscopy (FLIM) for the spectroscopic investigation of fluorescence properties of dyes in tissue section. It allows e.g. the multiplexing of stains in the presence of high tissue autofluorescence.