CRISPR/Cas13-mediated antiviral therapy against SARS-CoV-2 and its variants: a novel technology to target viral genome

Project leader: Prof. Dr. med. Elisabeth Zeisberg

Key area

  • Antiviral strategies: agents and vaccines, antibodies

Who is involved?

  • Prof. Dr. med. Elisabeth Zeisberg (Project leader, UMG)
  • Prof. Dr. med. Michael Zeisberg (UMG)
  • Prof. Dr. med. Gerd Hasenfuß (UMG)
  • PD Dr. Xingbo Xu (UMG)
  • Prof. Dr. Stefan Pöhlmann (DPZ)
  • Prof. Dr. Rabea Hinkel (DPZ)
  • Prof. Dr. Dr. Albert Osterhaus (TiHo)

What is the aim?

We have developed a CRISPR/Cas13 system consisting of CRISPR/Cas13 and various crRNAs directed against SARS-CoV-2, which destroys the viral genome and mRNA. Using adeno-associated viruses (AAV) as vehicles, the system leads to a more than 99% reduction in the infectivity of SARS-CoV-2 in cell culture. Within the framework of COFONI, the efficacy of different combinations of crRNAs against SARS-CoV-2 in the hamster model, the therapeutic time slot as well as the safety and effectiveness against other variants will now be investigated.

Which COFONI technology platform is involved?

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